Cell Technology/Fluoro MPO/500/FLMPO100
商品编号：
FLMPO100
品牌：
celltechnology
市场价：
¥8900.00
美元价：
4450.00
产品分类：
限制性内切酶
公司分类：
Restriction_Enzymes
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
- Description
- Additional Information
- Readable Documents
- Assay Principle
- Reviews
Key Benefits
- Can monitor multiple time points to follow kinetics.
- One-step, no wash assay.
- Adaptable for High Throughput format.
- Highly Sensitive.
- Applications – Fluorescent Plate Reader or Absorbance Plate Reader.
Additional information
| Kit Size | 500 |
|---|
- Detection of MPO activity in neutrophils and macrophages.
- Detection of PMN infiltration in tissue samples (inflammation and innate host defense mechanisms).
- Acute and chronic inflammatory disorders due to oxidative tissue damage.
- MPO activity in acute and chronic manifestations of cardiovascular disease.
Figure 1. MPO standard curve was serially diluted in 1X Reaction Buffer. Reaction cocktail (RC) was prepared as described (without EPO inhibitor). Next 50uL of MPO standard and 50uL of RC was added to individual well of a 96 well black plates. The plate was incubated at room and temperature in the dark. Data collected Ex:530nm Em:590nm
| Document Title |
| MPOProtocol |
| Fluoro MPO Datasheet |
| msds.fluoroMPO |
| Title | File | Link | Author(s) | Journal | Year; Edition:Pages |
| Use of a hanging-weight system for liver ischemic preconditioning in mice | http://ajpgi.physiology.org/cgi/content/abstract/294/6/G1431 | Hart, Much, Kohler et.al | Am J Physiol Gastrointest Liver Physiol | 294: G1431-G1440, 2008 | |
| Extracellular Adenosine Production by Ecto-5′-Nucleotidase Protects During Murine Hepatic Ischemic Preconditioning | http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WFX-4T4HP30-3&_user=10&_coverDate=11%2F30%2F2008&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_searchStrId=1245872620&_rerunOrigin=scholar.google&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=0348e5d389c76f43fa0ca609f91a05f7 | M. Hart, C. Much, I. Gorzolla, J. Schittenhelm, D. Kloor, G. Stahl, H. Eltzschig | Gastroenterology | Volume 135, Issue 5, Pages 1739-1750.e3 | |
| Inflammatory Biomarkers of Sulfur Mustard Analog 2-Chloroethyl Ethyl Sulfide–Induced Skin Injury in SKH-1 Hairless Mice | http://toxsci.oxfordjournals.org/cgi/content/abstract/108/1/194 | Neera Tewari-Singh, Sumeet Rana, Mallikarjuna Gu, Arttatrana Pal, David J. Orlicky, Carl W. White and Rajesh Agarwal | Toxicological Sciences | 2009 108(1):194-206 |
| Reference |
| Olsen, R. L. & Little, c. (1983) Biochem. J. 209, 781-787. |
| Nauseef, W. M., and Malech, H. L. (1986) Blood, 67, 1504-1507. |
| Andrews, P. C., Parnes, C., and Krinsky, N. I. (1984) Arch. Biochem. Biophys., 228, 439-442. |
| Mark B. Hampton, Anthony J. Kettle, and Christine C. Winterbourn. Inside the Neutrophil Phagosome: Oxidants, Myeloperoxidase, and Bacterial Killing. Blood, Vol. 92 No. 9 (November 1), 1998: pp. 3007-3017. |
| Andrews, P.C., Parnes, C. & Krinsky, N.I. (1984) Comparison of myeloperoxidase and hemi-myeloperoxidase with respect to catalysis, regulation, and bacterial activity. Arch. Biochem. Biophys. 228, 439–442. |
| Mingjie Zhou, Zhenjun Diwu, Nataliya Panchuk-Voloshina and Richard P. Haugland. A Stable Nonfluorescent Derivative of Resorufin for the Fluorometric Determination of Trace Hydrogen Peroxide: Applications in Detecting the Activity of Phagocyte NADPH Oxidase and Other Oxidases. Anal Biochem 253, 162 (1997). |
| J. G. Mohanty, Jonathan S. Jaffe, Edward S. Schulman and Donald G. Raible. A highly sensitive fluorescent micro-assay of H2O2 release from activated human leukocytes using a dihydroxyphenoxazine derivative. J. Immunol Methods 202, 133 (1997). |
| Tatyana V. Votyakova and Ian J. Reynolds. Membrane Potential dependent and -independent production of reactive oxygen species by rat brain mitochondria. J Neurochem 79, 266 (2001). |
| Chun Song, Abu B. Al-Mehdi, and Aron B. Fisher. An immediate endothelial cell signaling response to lung ischemia. Am J Physiol Lung Cell Mol Physiol 281, L993 (2001). |
| Samantha C. Richer and W.C.L. Ford. A critical investigation of NADPH oxidase activity in human spermatozoa. Mol Hum Reprod 7, 237 (2001). |
| William G. Gutheil, Miglena E. Stefanova and Robert A. Nicholas. Fluorescent Coupled Enzyme Assays for -Alanine: Application to Penicillin-Binding Protein and Vancomycin Activity Assays. Anal Biochem 287, 196 (2000). |
| Dominik Peus, Remus A. Vasa, Astrid Beyerle, Alexander Meves, Carsten Krautmacher and Mark R. Pittelkow. UVB Activates ERK1/2 and p38 Signaling Pathways via Reactive Oxygen Species in Cultured Keratinocytes. J Invest Dermatol 112, 751 (1999). |
| Tatyana V.Votyakova ,Ian J.Reynolds. Detection of hydrogen peroxide with Amplex Red:interference by NADH and reduced glutathione auto-oxidation. Archives of Biochemistry and Biophysics, 431: 138-144 (2004).. |
| Elizabeth Forbes, Tosei Murase, Ming Yang, Klaus I. Matthaei,*James J. Lee, Nancy A. Lee, Paul S. Foster, and Simon P. Hogan. Immunopathogenesis of Experimental Ulcerative Colitis Is Mediated by Eosinophil Peroxidase. The Journal of Immunology, 2004, 172:5664–5675. |
| Part# | Reagent | Temperature |
| Part# 4007 | Detection Reagent, 1 Vial | -20C |
| Part# 3002 | 10X Assay Buffer, 60mL | 2-8C |
| Part# 3012 | Hydrogen Peroxide, 1000µL of a Stabilized 3% Solution | 2-8C |
| Part# 6015 | Myeloperoxidase, 1 Vial at 30Units/mL | 2-8C |
品牌介绍
我们的第一个产品是一系列产品，可通过测量活性胱天蛋白酶和线粒体膜电位的丧失来检测凋亡细胞。我们扩大了目录，提供了高度特异性的半胱天冬酶检测产品，以及用于检测组织蛋白酶和ELISA产品的产品。我们致力于开发独特的高科技试剂，从而继续扩展我们的产品线。
