细胞技术/氟过氧化氢酶/500/FLOCAT100
商品编号：
FLOCAT100
品牌：
celltechnology
市场价：
¥5900.00
美元价：
2950.00
产品分类：
其它酶
公司分类：
Other_enzymes
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
- Description
- Additional Information
- Readable Documents
- Assay Principle
- Reviews
Key Benefits
- Sensitive Fluorescent Assay.
- Enzyme Positive Control included in kit.
- Can monitor multiple time points to follow kinetics.
- One-step, No Wash assay.
- Adaptable for High Throughput format.
- Monitors enzymatic activity.
- Applications – Fluorescent Plate Reader.
Additional information
| Kit Size | 500 |
|---|
Catalase is an antioxidant enzyme that catalyses the decomposition of hydrogen peroxide (H202) to water and oxygen. Catalase is ubiquitously expressed in mammalian and non-mammalian aerobic cells containing the cytochrome system. The enzyme has been isolated from various sources, including bacteria and plant cells (1-3). Catalase activity varies greatly from tissue to tissue. Highest activity is seen in liver and kidney, while lowest activity is seen in connective tissue (3). In eukarotic cells, catalase in concentrated in organelles called peroxisomes (4).
The production of hydrogen peroxide in eukaryotic cells is an end product result of various oxidases and superoxide dismutase reactions. Accumulation of H202 can result in cellular damage through oxidation of proteins, DNA and lipids thus resulting in cell death and mutagenisis (8-11). H202 role in oxidative stress related diseases have been widely studied (8,12).
The Fluoro Catalase detection kit is sensitive assay that utilizes a non – fluorescent detection reagent to detect H202 substrate left over from the catalase reaction (5-6).
| Document Title |
| Fluoro Catalase Protocol |
| Fluoro Catalase Datasheet |
| msds.FluoroCatalase |
| Title | File | Link | Author(s) | Journal | Year; Edition:Pages |
| Expression of HIF prolyl hydroxylase isozymes in growth plate chondrocytes: Relationship between maturation and apoptotic sensitivity | http://onlinelibrary.wiley.com/doi/10.1002/jcp.20873/full | S.P. Terkhorn, J. Bohensky, I.M. Shapiro, E. Koyama, V. Srinivas | Journal of Cellular Physiology | Volume 210, Issue 1, pages 257–265, January 2007- DOI: 10.1002/jcp.20873 | |
| Lethal and sublethal effects of the pyrethroid, bifenthrin, on grass shrimp (Palaemonetes pugio) and sheepshead minnow (Cyprinodon variegatus) | http://www.informaworld.com/smpp/content~db=all~content=a795388224 | Heather E. Harper; Paul L. Pennington; Jennifer Hoguet; Michael H. Fulton | Journal of Environmental Science and Health | Part B: Pesticides, Food Contaminants, and Agricultural Wastes Volume 43, Issue 6, 2008, Pages 476 – 483 DOI: 10.1080/03601230802174599 | |
| 3β-Hydroxysteroid-{Delta}24 Reductase Is a Hydrogen Peroxide Scavenger, Protecting Cells from Oxidative Stress-Induced Apoptosis | http://endo.endojournals.org/cgi/content/abstract/149/7/3267 | Xiuli Lu, Fukushi Kambe, Xia Cao, Yasuko Kozaki, Takahide Kaji, Takehisa Ishii and Hisao Seo | Endocrinology | Vol. 149, No. 7 3267-3273, 2008 | |
| Regulation of autophagy in human and murine cartilage: Hypoxia-inducible factor 2 suppresses chondrocyte autophagy | http://onlinelibrary.wiley.com/doi/10.1002/art.24444/full?globalMessage=0 | Jolene Bohensky1, Shawn P. Terkhorn1, Theresa A. Freeman1, Christopher S. Adams1, Joseph A. Garcia2, Irving M. Shapiro1, Vickram Srinivas | Arthritis and Rheumatism | Vol 60, Issue 5, pp 1406-1415, May 2009. DOI: 10.1002/art.24444 |
| Reference |
| Deisseroth, A., and Dounce, A.L. Catalase: Physical and chemical properties, mechanism of catalysis, and physiological role Physiol. Rev., 50, 319-375 (1970). |
| Sebastian Mueller, Hans-Dieter Riedel and Wolfgang Stremmel. Determination of Catalase Activity at Physiological Hydrogen Peroxide Concentrations. Analytical Biochemistry Volume 245, Issue 1 ,1 February 1997, Pages 55-60. |
| Deisseroth, A., and Dounce, A.L. Catalase: Physical and chemical properties, mechanism of catalysis, and physiological role. Physiol. Rev., 50, 319-375 (1970). |
| Marcel Zámocký and Franz Koller . Understanding the structure and function of catalases: clues from molecular evolution and in vitro mutagenesis. Progress in Biophys. Mol. Biol., 72, 19-66 (1999). |
| Mingjie Zhou, Zhenjun Diwu, Nataliya Panchuk-Voloshina and Richard P. Haugland. A Stable Nonfluorescent Derivative of Resorufin for the Fluorometric Determination of Trace Hydrogen Peroxide: Applications in Detecting the Activity of Phagocyte NADPH Oxidase and Other Oxidases. Anal Biochem 253, 162 (1997). |
| J. G. Mohanty, Jonathan S. Jaffe, Edward S. Schulman and Donald G. Raible. A highly sensitive fluorescent micro-assay of H202 release from activated human leukocytes using a dihydroxyphenoxazine derivative. J. Immunol Methods 202, 133 (1997). |
| Tatyana V.Votyakova ,Ian J.Reynolds. Detection of hydrogen peroxide with Amplex Red:interference by NADH and reduced glutathione auto-oxidation. Archives of Biochemistry and Biophysics, 431:138-144 (2004). |
| Jingxiang Bai, Ana M. Rodriguez, J. Andres Melendez, and Arthur I. Cederbaum. Overexpression of Catalase in Cytosolic or Mitochondrial Compartment Protects HepG2 Cells against Oxidative Injury. J. Biol. Chem., Sep 1999; 274: 26217 - 26224 |
| Tada-Oikawa, S. et al., FEBS Lett., 442, 65-69 (1999). |
| Hampton, M.B., and Orrenius, S., FEBS Lett., 414, 552-556 (1997). |
| Kowaltowski, A.J. et al., FEBS Lett., 473, 177-182 (2000). |
| Tome, M.E. et al., Cancer Res., 61, 2766-2733 (2001). |
| Part# | Reagent | Temperature |
| 3021 | 5X Reaction Buffer pH 7.4, 25mL | 2-8C |
| 4010 | Detection Reagent, 1 Vial | 2-8C - Undiluted; Diluted: -20C(Aliquot in Single Use Vials) |
| 6009 | Horseradish Peroxidase, 1 Vial | 2-8C - Undiluted; Diluted: -20C |
| 3022 | Hydrogen Peroxide, 1 Vial | 2-8C |
| 6008 | Catalase Enzyme, 1 Vial | 2-8C (Crystalline Suspension) |
品牌介绍
我们的第一个产品是一系列产品，可通过测量活性胱天蛋白酶和线粒体膜电位的丧失来检测凋亡细胞。我们扩大了目录，提供了高度特异性的半胱天冬酶检测产品，以及用于检测组织蛋白酶和ELISA产品的产品。我们致力于开发独特的高科技试剂，从而继续扩展我们的产品线。
