Cell Technology/氟羟基/过氧亚硝酸盐/150/FLHPF100
商品编号：
FLHPF100
品牌：
celltechnology
市场价：
¥5100.00
美元价：
2550.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
- Description
- Additional Information
- Readable Documents
- Assay Principle
- Reviews
Introduction
A new novel probe, Hydroxyphenyl fluorescein (HPF), developed by Tetsuo Nagano et. al. (1), is a highly selective probe for the detection of highly Reactive Oxygen Species (hROS). It is a cell permeable highly sensitive fluorescent probe for hydroxyl radical (OH•), and peroxynitrite (ONOO-) detection. It has little reactivity towards other hROS such as: hypochlorite (-OCl), singlet oxygen (021), superoxide (02-•), hydrogen peroxide (H202), nitric oxide (NO•), and alkyl peroxide (RO2•) (see table below)1.
Key Benefits
- Quenched Cell permeable dye.
- One Step, No wash Homogenous assay.
- Adaptable to High throughput assay platforms.
- Can monitor multiple time points to follow real time kinetics.
- Non-destructive cell based assay allows monitoring of additional parameters.
- Applications – Fluorescent plate reader / Flow Cytometery / Fluorescent Microscopy.
Additional information
| Kit Size | 150 |
|---|
| Document Title |
| HPFProtocol |
| Fluoro HPF Datasheet |
| msds.APF-HPF-OCl |
| Title | File | Link | Author(s) | Journal | Year; Edition:Pages |
| Paradoxical effect of l-arginine: Acceleration of endothelial cell senescence | http://www.sciencedirect.com/science/article/pii/S0006291X09012376 | Ortunato Scalera, Ellen I. Closs, Eva Flick, Jens Martens-Lobenhoffer, Jean P. Boissel, Uwe Lendeckel, Anke Heimburg and Stefanie M. Bode-Böger | Biochemical and Biophysical Research Communications | Volume 386, Issue 4, 4 September 2009, Pages 650-655 | |
| The neuroprotective effect of ladostigil against hydrogen peroxide-mediated cytotoxicity | http://www.sciencedirect.com/science/article/pii/S0009279708003086 | Orly Weinreb, Orit Bar-Am, Tamar Amit, Noam Drigues, Yotam Sagi and Moussa B.H. Youdim | Chemico-Biological Interactions | Volume 175, Issues 1-3, 25 September 2008, Pages 318-326 | |
| Generation of highly-reactive oxygen species is closely related to hair cell damage in rat organ of corti treated with gentamicin | http://www.sciencedirect.com/science/article/pii/S0306452209003352 | Y.H. Choung, A. Taura, K. Pak, S.J. Choi, M. Masuda and A.F. Ryan | Neuroscience | Volume 161, Issue 1, 16 June 2009, Pages 214-226 | |
| The Novel Cholinesterase–Monoamine Oxidase Inhibitor and Antioxidant, Ladostigil, Confers Neuroprotection in Neuroblastoma Cells and Aged Rats | http://www.springerlink.com/content/tgg06484h1142777/ | Orit Bar-Am, Orly Weinreb, Tamar Amit and Moussa B. H. Youdim | Journal of Molecular Neuroscience | Volume 37, Number 2, 135-145, DOI: 10.1007/s12031-008-9139-6 | |
| Post-translational activation of human phenylalanine 4-monooxygenase from an endobiotic to a xenobiotic enzyme by reactive oxygen and reactive nitrogen species | http://informahealthcare.com/doi/abs/10.3109/00498251003675207 | A. Antypa, C. Rebello, A. Biernacka, K. Krajewski, J. Cassam, S. C. Mitchell, G. B. Steventon | doi:10.3109/00498251003675207 | May 2010, Vol. 40, No. 5 , Pages 319-330 |
| Reference |
| Ken-ichi Setsukinai, Yasuteru Urano, Katsuko Kakinuma, Hideyuki J. Majima , and Tetsuo Nagano. Development of Novel Fluorescence Probes That Can Reliably Detect Reactive Oxygen Species and Distinguish Specific Species. THE JOURNAL OF BIOLOGICAL CHEMISTRY Vol. 278, No. 5, Issue of January 31, pp. 3170–3175, 2003 |
| Part# | Reagent | Temperature |
| Part# 4012 | HPF, 1 Vial | 2-8C |
品牌介绍
我们的第一个产品是一系列产品，可通过测量活性胱天蛋白酶和线粒体膜电位的丧失来检测凋亡细胞。我们扩大了目录，提供了高度特异性的半胱天冬酶检测产品，以及用于检测组织蛋白酶和ELISA产品的产品。我们致力于开发独特的高科技试剂，从而继续扩展我们的产品线。
