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Phospho-Tyrosine Mouse mAb (P-Tyr-100) Beads (for use with...
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发布时间：2024-05-14
Phospho-Tyrosine Mouse mAb (P-Tyr-100) Beads (for use with PhosphoScan® Technology)
Cellular Assay Kits
Assay Kit
Phospho-Tyrosine Mouse mAb (P-Tyr-100) Beads (for use with PhosphoScan® Technology)#7902
Phospho-Tyrosine Mouse mAb (P-Tyr-100) Beads (for use with PhosphoScan® Technology) 7902
Product Description Cell Signaling Technology\'s patented PhosphoScan® Technology allows for the purification and identification of phosphorylation sites in cellular proteins when coupled with LC tandem mass spectrometry. In this assay, PhosphoScan® (P-Tyr-100) mAb Beads are used to specifically enrich phospho-tyrosine-containing peptides. Cells are lysed in a urea-containing buffer, and cellular proteins are digested by proteases and fractionated by reversed-phase solid-phase extraction. Peptides are then subjected to immunoaffinity purification using PhosphoScan® (P-Tyr-100) mAb Beads. Overnight incubation ensures high-affinity binding of phospho-tyrosine-containing peptides to P-Tyr-100 beads. Unbound peptides are removed through washing, and phospho-tyrosine-containing peptides are eluted with dilute acid. Reversed-phase chromatography is performed on microtips to separate phosphopeptides from antibody and to concentrate them for LC tandem mass spectrometry.
Background PTMScan® Technology employs a proprietary methodology from Cell Signaling Technology for peptide enrichment by immunoprecipitation using a specific bead-conjugated antibody in conjunction with liquid chromatography (LC) tandem mass spectrometry (MS/MS) for quantitative profiling of post-translational modification (PTM) sites in cellular proteins. These include phosphorylation (PhosphoScan®), ubiquitination (UbiScan®), acetylation (AcetylScan®), and methylation (MethylScan®), among others. PTMScan® enables researchers to isolate, identify and quantitate large numbers of post-translationally modified cellular peptides with a high degree of specificity and sensitivity providing a global overview of PTMs in cell and tissue samples without preconceived biases about where these modified sites occur. For more information on PTMScan® services, please visit www.cellsignal.com/services/index.html Tyrosine phosphorylation plays a key role in cellular signaling (2). In cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals (3). Current phosphoproteomic approaches generally reveal only small numbers of tyrosine phosphorylation sites, in keeping with the low level of phosphotyrosine relative to phosphoserine and phosphothreonine residues (4). PhosphoScan® Kit, based on CST\'s published method (1,5), isolates large numbers of cellular phosphotyrosine-containing peptides. This allows the investigator to obtain a global overview of tyrosine phosphorylation in the cell, covering many classes of proteins without preconceived biases about where tyrosine phosphorylation sites will be found. Schlessinger, J. (2000) Cell 103, 211-25.
除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。 专品专有“专供研究使用”的专专或专似的专专声明， 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的， 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的， 或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专 For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. PhosphoScan is a trademark of Cell Signaling Technology, Inc. Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at [email protected] For information regarding commercial licensing terms please contact CST Pharma Services Department at [email protected]
Phospho-Tyrosine Mouse mAb (P-Tyr-100) Beads (for use with PhosphoScan® Technology)#7902
Phospho-Tyrosine Mouse mAb (P-Tyr-100) Beads (for use with PhosphoScan® Technology) 7902
Product Description Cell Signaling Technology\'s patented PhosphoScan® Technology allows for the purification and identification of phosphorylation sites in cellular proteins when coupled with LC tandem mass spectrometry. In this assay, PhosphoScan® (P-Tyr-100) mAb Beads are used to specifically enrich phospho-tyrosine-containing peptides. Cells are lysed in a urea-containing buffer, and cellular proteins are digested by proteases and fractionated by reversed-phase solid-phase extraction. Peptides are then subjected to immunoaffinity purification using PhosphoScan® (P-Tyr-100) mAb Beads. Overnight incubation ensures high-affinity binding of phospho-tyrosine-containing peptides to P-Tyr-100 beads. Unbound peptides are removed through washing, and phospho-tyrosine-containing peptides are eluted with dilute acid. Reversed-phase chromatography is performed on microtips to separate phosphopeptides from antibody and to concentrate them for LC tandem mass spectrometry.
Background PTMScan® Technology employs a proprietary methodology from Cell Signaling Technology for peptide enrichment by immunoprecipitation using a specific bead-conjugated antibody in conjunction with liquid chromatography (LC) tandem mass spectrometry (MS/MS) for quantitative profiling of post-translational modification (PTM) sites in cellular proteins. These include phosphorylation (PhosphoScan®), ubiquitination (UbiScan®), acetylation (AcetylScan®), and methylation (MethylScan®), among others. PTMScan® enables researchers to isolate, identify and quantitate large numbers of post-translationally modified cellular peptides with a high degree of specificity and sensitivity providing a global overview of PTMs in cell and tissue samples without preconceived biases about where these modified sites occur. For more information on PTMScan® services, please visit www.cellsignal.com/services/index.html Tyrosine phosphorylation plays a key role in cellular signaling (2). In cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals (3). Current phosphoproteomic approaches generally reveal only small numbers of tyrosine phosphorylation sites, in keeping with the low level of phosphotyrosine relative to phosphoserine and phosphothreonine residues (4). PhosphoScan® Kit, based on CST\'s published method (1,5), isolates large numbers of cellular phosphotyrosine-containing peptides. This allows the investigator to obtain a global overview of tyrosine phosphorylation in the cell, covering many classes of proteins without preconceived biases about where tyrosine phosphorylation sites will be found. Schlessinger, J. (2000) Cell 103, 211-25.
除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。 专品专有“专供研究使用”的专专或专似的专专声明， 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的， 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的， 或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专 For Research Use Only. Not For Use In Diagnostic Procedures. Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc. PhosphoScan is a trademark of Cell Signaling Technology, Inc. Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at [email protected] For information regarding commercial licensing terms please contact CST Pharma Services Department at [email protected]
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发布于 ： 2024-05-14
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